tubes collecting bal fluid samples  (Eppendorf AG)

Journal: Open Forum Infectious Diseases

Article Title: An Observational Cohort Study of Bronchoalveolar Lavage Fluid Galactomannan and Aspergillus Culture Positivity in Patients Requiring Mechanical Ventilation

doi: 10.1093/ofid/ofaf090

Figure Lengend Snippet: Bronchoalveolar lavage (BAL) fluid galactomannan and Aspergillus growth. A , Summary on a patient level. B , Summary of BAL samples. Abbreviations: BAL, bronchoalveolar lavage; GM, galactomannan; ODI, optical density index.

Article Snippet: These samples are typically sent for cell count and differential, amylase, bacterial and fungal cultures (incubated for 4 weeks at 30 ° C on inhibitory mold agar plates with identification performed by microscopic morphology and ability to grow at 45 ° C), BAL fluid GM (Bio-Rad Platelia Aspergillus Ag), and multiplex polymerase chain reaction (PCR; both viral and bacterial), as well as other studies when indicated.

Techniques:

Journal: Open Forum Infectious Diseases

Article Title: An Observational Cohort Study of Bronchoalveolar Lavage Fluid Galactomannan and Aspergillus Culture Positivity in Patients Requiring Mechanical Ventilation

doi: 10.1093/ofid/ofaf090

Figure Lengend Snippet: A , Bronchoalveolar lavage (BAL) fluid galactomannan (GM) optical density index (ODI) is higher in patients who grow Aspergillus at least once during admission. B , BAL fluid GM ODI is higher in the BAL samples where Aspergillus grew, though sometimes also elevated in BAL samples that did not grow Aspergillus .

Article Snippet: These samples are typically sent for cell count and differential, amylase, bacterial and fungal cultures (incubated for 4 weeks at 30 ° C on inhibitory mold agar plates with identification performed by microscopic morphology and ability to grow at 45 ° C), BAL fluid GM (Bio-Rad Platelia Aspergillus Ag), and multiplex polymerase chain reaction (PCR; both viral and bacterial), as well as other studies when indicated.

Techniques:

Journal: Open Forum Infectious Diseases

Article Title: An Observational Cohort Study of Bronchoalveolar Lavage Fluid Galactomannan and Aspergillus Culture Positivity in Patients Requiring Mechanical Ventilation

doi: 10.1093/ofid/ofaf090

Figure Lengend Snippet: Example timelines of four patients ( A , B , C , D ) who grew Aspergillus on bronchoalveolar lavage (BAL) fluid culture (red square indicates Aspergillus growth, blue circle indicates no Aspergillus growth) and BAL fluid galactomannan (GM) optical density index (ODI) levels (y-axis). Patients who grew Aspergillus did not grow it consistently, even on BAL samples that had elevated BAL fluid GM ODIs. The bottom line of each graph shows antifungal therapy with anti- Aspergillus activity by intensive care unit (ICU) day (gray x marker, no antifungal therapy; green plus marker, antifungal therapy administered).

Article Snippet: These samples are typically sent for cell count and differential, amylase, bacterial and fungal cultures (incubated for 4 weeks at 30 ° C on inhibitory mold agar plates with identification performed by microscopic morphology and ability to grow at 45 ° C), BAL fluid GM (Bio-Rad Platelia Aspergillus Ag), and multiplex polymerase chain reaction (PCR; both viral and bacterial), as well as other studies when indicated.

Techniques: Activity Assay, Marker